Dose at 150 grams per 1,000 litres of culture. 1.5 kilograms makes 10,000 litres at Walnes strength, so divide your culture volume in litres by 10,000 and multiply by 1.5 to get the kilograms per batch.

Cell-Hi Walnes Algae Nutrient

Q: Which algae does Cell-Hi Walnes suit?

It is particularly suitable for flagellate algae such as Isochrysis and Tetraselmis, and is also used for Chlorella vulgaris, Nannochloropsis, Isochrysis galbana, Dunaliella salina and Skeletonema.

Q: Should I choose Walnes or Cell-Hi F2P?

Walnes is the flagellate formulation. Cell-Hi F2P follows the Guillard F/2 recipe and is the usual choice for diatoms such as Chaetoceros calcitrans, Chaetoceros muelleri and Thalassiosira pseudonana.

Q: How do I make up a stock solution?

Formulate the stock at 1.5 kilograms per 10 litres and use it at 1 millilitre per litre of culture. Sterilise with a 0.2 micron syringe filter or by autoclaving.

Q: Do I need to add silicate?

Yes for diatoms. Add 30 grams of sodium metasilicate for each 1,000 litres of culture water after adding the Cell-Hi.

Q: Can I dose harder in a photobioreactor?

Yes. For advanced and tubular reactors giving much higher algal concentrations, multiply the dose rate by two to four times if required.

Q: How should it be stored?

Store it in a cool, dark place away from direct sunlight and high temperatures so the nutrients stay stable and effective.

A nutrient concentrate for culturing microalgae as live feed in hatcheries and algae production systems.

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Categories: Algae Systems, Bacteria & Additives, Hatchery

Applications: Hatchery, RAS

Brand: Varicon Aqua

Product Information

Key Features

Walnes formulation algal nutrient, recommended by the UK Culture Collection for algae, protozoa and flagellates
Particularly suited to flagellates such as Isochrysis and Tetraselmis, and used for Chlorella vulgaris, Nannochloropsis, Isochrysis galbana, Dunaliella salina and Skeletonema
Supplies nitrogen, phosphorus and magnesium with iron, zinc, manganese, copper, molybdenum, cobalt and boron, plus B12, thiamine and biotin
Dosed at 0.15 g/L — 150 g per 1,000 L of culture — for both batch and continuous cultivation, and suits systems running at pH 7.5–8.5
Made up as a stock at 1.5 kg per 10 L and used at 1 mL per litre of culture
Advanced and tubular reactors at higher cell densities can be run at 2 to 4 times the dose rate
For diatoms, add 30 g of sodium metasilicate per 1,000 L of culture water after the Cell-Hi; shipped as Dangerous Goods, so ask us for freight
Sizing — 1.5 kg makes 10,000 L of medium at Walnes strength, so divide your culture volume in litres by 10,000 and multiply by 1.5 to get the kilograms per batch, then choose the 1 kg unit or the 20 kg case

Specifications

Specification	Detail	SKU
Pack size	1 kg	WP
Pack size	Case of 20 x 1 kg	WPC
Form	Soluble powder
Formulation	Walnes medium — recommended by the UK Culture Collection for algae, protozoa and flagellates
Dose rate	0.15 g/L — 150 g per 1,000 L of culture, for both batch and continuous cultivation
Coverage	1.5 kg makes 10,000 L of culture medium at Walnes strength
Stock solution	Formulate at 1.5 kg per 10 L and use at 1 mL per litre of culture
High-density reactors	For advanced and tubular reactors, multiply the dose rate by 2 to 4 times if required
Diatoms	Add 30 g of sodium metasilicate per 1,000 L of culture water after the Cell-Hi
Essential nutrients	Nitrogen, phosphorus and magnesium, with iron, zinc, manganese, copper, molybdenum, cobalt and boron
Vitamins	B12, thiamine and biotin
Optimal pH range	7.5–8.5
Species suited	Particularly flagellates such as Isochrysis and Tetraselmis; also Chlorella vulgaris, Nannochloropsis, Isochrysis galbana, Dunaliella salina and Skeletonema
Sterilisation	0.2 micron syringe filter or autoclaving
Storage	Cool, dark place away from direct sunlight and high temperatures
Freight	Shipped as Dangerous Goods — contact Fresh By Design for shipping costs
Manufacturer	Varicon Aqua

Downloads

Cell-Hi Walnes Technical Sheet Cell-Hi Instructions

Frequently Asked Questions

How much do I need?

Which algae does Cell-Hi Walnes suit?

Should I choose Walnes or Cell-Hi F2P?

How do I make up a stock solution?

Do I need to add silicate?

Can I dose harder in a photobioreactor?

How should it be stored?

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Cell-Hi Walnes Algae Nutrient

Product Information

Key Features

Specifications

Downloads

Frequently Asked Questions

Spare Parts & Consumables

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